Uber has 21 days to appeal the decision and meet TfL's standards.
Uber's license to operate as a private hire service is set to expire on September 30.
In one of the fastest growing petitions with a record number of people signing Uber's petition against Transport for London's decision to strip the firm of their licence, Uber has received more than half a million signatures in just over 24 hours.
The Mayor of London advised more than 500,000 people protesting the decision to ban taxi service Uber from the capital to turn their anger on the company for its failure to address safety and security issues.
London Mayor Sadiq Khan said he supported Friday's decision, saying any operator of taxi services in the city "needs to play by the rules".
However, a campaign Save Uber in London has become the fastest growing petition in the United Kingdom this year.
Some 400,000 people have signed a petition urging Transport for London (TfL) to reverse its decision not to renew Uber's licence, the media reported on Saturday.
The ubiquitous auto share service has experienced explosive growth in dense and sprawling London, where an estimated 40,000 Uber drivers prowl the streets waiting for a click from the 3.5 million residents who have downloaded the app.
"Leeds City Council has put in place some of the most challenging policies and conditions in the United Kingdom to ensure public and taxi passenger safety, with different regulations in Leeds compared to London which mean the safety and security issues raised by TfL are not applicable in Leeds", a spokesman said. However, Tom Elvidge, General Manager of Uber in London, said in a statement that an "independent review found that "greyball" has never been used or considered in the United Kingdom".
"We intend to immediately challenge this in the courts".
"Not only will this decision deprive you of the choice of a convenient way of getting about town, it will also put more than 40,000 licensed drivers who rely on our app out of work".
And we are joined by Rosh Varma who drives a black taxi and is a member of the United Cabbies Group, and Syed Khalil who now works as a driver for Uber.
Last month, Uber was accused by police of allowing a driver who sexually assaulted a passenger to strike again by not reporting the attack, along with other serious crimes.
The company pulled out of Austin, Texas, when it was told its drivers would have to undergo fingerprint background checks, but resumed services after the requirement was ended.