The Russian Defense Ministry first posted the alleged proof on Tuesday morning, with one black-and-white image showing a top-down gunship view of a series of trucks supposedly leaving the eastern ISIS-held village of Al-Bukamal for the nearby Syria-Iraq border.
At least one of the photographs exactly matched a frame from a promotional video for a "AC-130 Gunship Simulator" computer game posted online in March 2015.
Russia's Defense Ministry says it has launched an inquiry into pictures mistakenly attached to its statement.
"I certainly can't verify but I have seen a report that one of the pictures came from a video game".
The images were later deleted from the ministry of defense's Twitter and Facebook accounts, and the ministry said several hours later that there had been a "mistake", and published a different set of pictures, calling them "irrefutable proof" of United States aid of IS.
In the corner of the image, however, a few letters of the developer's disclaimer can still be seen: "Development footage". This is a work in progress. The statement wasn't even fully cropped out of the images that Russian Federation tweeted.
Earlier today, the ministry tweeted three times in various languages, with a series of pictures accusing the USA of co-operation with the so-called Islamic state.
Asked about Russian allegations of the USA helping Islamic State around Albu Kamal, Colonel Ryan Dillon, spokesman for the US -led coalition fighting Islamic State, said: "The Russian ministry of defence statements are about as accurate as their air campaign and I think that is a reason for them to start, you know, coming out with their latest barrage of lies".
It said the US-led coalition refused requests to cooperate and "eliminate fleeing Isis convoys".
The Russian military is investigating claims that a civilian employee attached the bogus images, state-run media reported later Tuesday.
Responding to Russia's allegations in remarks carried by Reuters, a spokesman for the US-led coalition Col Ryan Dillon said the Russian allegations were "about as accurate as their air campaign".